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黄新祥



联系方式:Email: huxinx@ujs.edu.cn 电话:0511-88780167 


个人简介

教授,博士生导师,医学院副院长,中国微生物学学会分析微生物学专业委员会委员,江苏省“333高层次人才培养工程”中青年科学技术带头人。1985年毕业于苏州医学院获医学学士学位,1991年大连医学院研究生毕业获生物化学硕士学位,2004年于日本岐阜大学微生物学与生物信息学博士研究生毕业并获日本(甲类)医学博士学位。


主讲课程

《医学生物化学》(本科)、《高级生物化学》(硕士研究生)、《Biochemistry and Molecular Biology》(MBBS留本科学生)、《Advance in Biochemistry and Molecular Biology》(留学研究生)。


近期主要研究方向: 

近年来主要从事有关人类肠道致病菌基因表达研究,首次发现伤寒沙门菌的z66鞭毛抗原的编码基因并揭示其具有二相鞭毛基因的分子遗传学特性;建立了伤寒沙门菌全基因组DNA芯片分析系统,并应用于伤寒沙门菌高渗应激下基因组表达变化研究,发现并验证了PhoP、OmpR、Mig14,RpoE与RpoS对伤寒沙门菌侵袭相关的毒性基因表达调节作用;利用高通量序列分析结合分子细菌学方法,发现并验证了一系列新的沙门菌非编码RNA,并揭示其在沙门菌中的功能和作用特性。


近期获得主要科研课题

1.长链反义RNA 调控伤寒沙门菌毒力机制研究(81371780)(2014.1-2017.12),国家自然科学基金(主持);

2.抗H:z66 抗体诱导伤寒沙门菌鞭毛相变换机制(30570088)(2009.1-2011.12),国家自然科学基金(主持);

3.病原体变异及其生物信息学分析技术研究(2008ZX10402)(2008.10-2011.12),国家科技重大专项课题(子课题负责);

4.重要食源性致病菌的标识基因和基因差异表达的研究(2006BAK02A15-2)(2006.1-2008.12),国家科技支撑计划“食品安全关键技术”重大项目子项目(子课题负责);

5.伤寒沙门菌z66 鞭毛抗原表达调节机制研究(30570088)(2006.1-2008.12),国家自然科学基金(主持);

6.伤寒沙门菌z66鞭毛抗原表达调节机制研究(2005.1-2006.12),教育部回国人员科研启动基金(主持)。

 

近期发表的主要SCI论文(*为通讯作者)

1.Zhang Q, Zhang Y, Zhang X, Zhan L, Zhao X, Xu S, Sheng X, Huang X*. The novel cis-encoded antisense RNA AsrC positively regulates the expression of rpoE-rseABC operon and thus enhances the motility of Salmonella enterica serovar Typhi. Front Microbiol. 2015, 17; 6: 990.

2.Gong M, Xu S, Jin Y, Zhang Y, Dadzie I, Zhang X, Wang Z, Zhu Y, Ni B, Sheng X, Huang X*. 5'-UTR of malS increases the invasive capacity of Salmonella enterica serovar Typhi by influencing the expression of bax. Future Microbiol. 2015 Jun, 10:941-54.

3.Zheng X, Ji Y, Weng X, Huang X*. RpoS-Dependent Expression of OsmY in Salmonella enterica serovar Typhi: Activation Under Stationary Phase and SPI-2-Inducing Conditions. Curr Microbiol. 2015 Jun, 70(6):877-82.

4.Dadzie I, Ni B, Gong M, Ying Z, Zhang H, Sheng X, Xu S, Huang X*. Identification and characterization of a cis antisense RNA of the parC gene encoding DNA topoisomerase IV of Salmonella enterica serovar Typhi. Res Microbiol. 2014 Jul-Aug, 165(6):439-46. 

5.Zhao X, Zhu Y, Zhang H, Du H, Ni B, Huang X*. Transcriptional expression of six genes located on pBSSB1 of Salmonella enterica serovar Typhi in different growth phases and environmental stresses. Curr Microbiol. 2014 Sep;69(3):252-7.

6.Sheng X, Xu S, Lu R, Isaac D, Zhang X, Zhang H, Wang H, Qiao Z, Huang X*. Design of a comprehensive biochemistry and molecular biology experiment: phase variation caused by recombinational regulation of bacterial gene expression. Biochem Mol Biol Educ. 2014 May-Jun;42(3):224-9.

7.Sheng X, Zhang H, Xia Q, Xu S, Xu H, Huang X*. Mig-14 plays an important role in influencing gene expression of Salmonella enterica serovar Typhi, which contributes to cell invasion under hyperosmotic conditions. Res Microbiol. 2013, 164 : 903-912.

8.Dadzie I, Xu S, Ni B, Zhang X, Zhang H, Sheng X, Xu H, Huang X*. Identification and Characterization of a Cis-Encoded Antisense RNA Associated with the Replication Process of Salmonella enterica Serovar Typhi. PLoS One. 2013, 8: e61308.

9.Zhang H, Ni B, Zhao X, Dadzie I, Du H, Wang Q, Xu H, Huang X*. Fis is essential for the stability of linear plasmid pBSSB1 and affects the motility of Salmonella enterica Serovar Typhi. PLoS One. 2012, 7: e37462.

10.Zhang H, Du H, Ji X, Ni B, Mao L, Xu S, Sheng X, Xu H, Huang X*. OmpR may regulate the putative YehU/YehT two-component system in Salmonella enterica serovar Typhi under hypotonic growth condition. Curr Microbiol. 2012, 64: 283-289.

11.Luo Z, Wang M, Du H, Yang T, Wang F, Ni B, Xu S, Huang X*. The putative sensor kinase QseC of Salmonella enterica serovar Typhi can promote invasion in the presence of glucose. Food Research International. 2012, 45: 1004–1010.

12.Zhang H, Zhang X, Yan M, Pang B, Kan B, Xu H, Huang X*. Genotyping of Salmonella enterica serovar Typhi strains isolated from 1959 to 2006 in China and analysis of genetic diversity by genomic microarray. Croat Med J. 2011, 52: 688-693.

13.Du H, Sheng X, Zhang H, Zou X, Ni B, Xu S, Zhu X, Xu H, Huang X*. RpoE may promote flagellar gene expression in Salmonella enterica serovar typhi under hyperosmotic stress. Curr Microbiol. 2011, 62: 492-500.

14.Du H, Wang M, Luo Z, Ni B, Wang F, Meng Y, Xu S, Huang X*. Coregulation of gene expression by sigma factors RpoE and RpoS in Salmonella enterica serovar Typhi during hyperosmotic stress. Curr Microbiol. 2011, 62: 1483-1489.

15.Wang M, Luo Z, Du H, Xu S, Ni B, Zhang H, Sheng X, Xu H, Huang X*. Molecular characterization of a functional type VI secretion system in Salmonella enterica serovar Typhi. Curr Microbiol. 2011, 63: 22-31.

16.Gao H, Zhang Y, Yang L, Liu X, Guo Z, Tan Y, Han Y, Huang X, Zhou D*, Yang R*. Regulatory effects of cAMP receptor protein (CRP) on porin genes and its own gene in Yersinia pestis. BMC Microbiol. 2011, 11: 40.

17.Gao H, Zhang Y, Han Y, Yang L, Liu X, Guo Z, Tan Y, , Huang X, Zhou D*, Yang R*. Phenotypic and transcriptional analysis of the osmotic regulator OmpR in Yersinia pestis. 2011, 11: 39.

18.Xie X, Li A, Du H, Sheng X, Zhang H, Xu S, Huang X*. Expression of tviA is transiently repressed by Hfq in Salmonella enterica serovar Typhi at hyperosmotic stress. Microb Pathog. 2010, 49: 54-57.

19.Xu S, Zou X, Sheng X, Zhang H, Mao L, Du H, Xu H, Huang X*. Expression of fljB:z66 on a linear plasmid of Salmonella enterica serovar Typhi is dependent on FliA and FlhDC and regulated by OmpR. Br J Microbiol. 2010, 41: 729-740.

20.Zou X, Huang X*, Xu S, Zhou L, Sheng X, Zhang H, Xu H, Ezaki T. Identification of a fljA gene on a linear plasmid as the repressor gene of fliC in Salmonella enterica serovar Typhi. Microbiol Immunol. 2009, 53: 191-197.

21.Sheng X, Huang X*, Li J, Xie X, Xu S, Zhang H, Xu H. Regulation of sulfur assimilation pathways in Salmonella enterica serovar Typhi upon up-shift high osmotic treatment: the role of UhpA revealed through transcriptome profiling. Curr Microbiol. 2009, 59: 628-635.

22.Zhang H, Sheng X, Xu S, Gao Y, Du H, Li J, Xu H, Huang X*. Global transcriptional response of Salmonella enterica serovar Typhi to anti-z66 antiserum. FEMS Microbiol Lett. 2009, 298: 51-55.

23.Sheng X, Huang X*, Mao L, Zhu C, Xu S, Zhang H, Xu H, Liu X. Preparation of genomic DNA microarray of Salmonella enterica serovar Typhi for gene expression profile analysis. Prog Biochem Biophs. 2009, 36: 206-212. 

24.Xu S, Zhang H, Sheng X, Xu H, Huang X*. Transcriptional expression of fljB:z66, a flagellin gene located on a novel linear plasmid of Salmonella enterica serovar Typhi under environmental stresses. New Microbiol. 2008, 31: 241-247.

25.Xu S, Huang X, Xu H, Zhang C*. Improved prediction of coreceptor usage and phenotype of HIV-1 based on combined features of V3 loop sequence using random forest. J Microbiol. 2007, 45: 441-446.

26.Huang X, Xu H, Sun X, Ohkusu K, Kawamura Y, Ezaki T*. Genome-Wide scan of the gene expression kinetics of Salmonella enterica serovar Typhi during hyperosmotic stress. Int J Mol Sci. 2007, 8: 116-135.

27.Huang X, Xu H, Sha MM, Zhao L, Ohkusu K, Kawamura Y, Ezaki T*. Virulence-defective strains of Salmonella enterica serovar Typhi as candidates for education at level 2 facilities. Microbiol Immunol. 2006, 50: 273-279.

28.Huang X, Phung le V, Dejsirilert S, Tishyadhigama P, Li Y, Liu H, Hirose K, Kawamura Y, Ezaki T*. Cloning and characterization of the gene encoding the z66 antigen of Salmonella enterica serovar Typhi. FEMS Microbiol Lett. 2004, 234: 239-246.